Molecular Cytogenetics: Protocols and Applications (Methods in Molecular Biol

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Molecular Cytogenetics: Protocols and Applications (Methods in Molecular Biology)
Author: Yao-Shan Fan (Editor)
Product Details
575 pages, Humana Press (October 2002) $135.00
Format: 4.24MB PDF



Summary:
Yao-Shan Fan and a panel of senior scientists and pioneering researchers describe in step-by-step style the leading FISH techniques and those molecular technologies beyond FISH available for diagnostic services in genetics and oncology. The methods include labeling FISH probes for DNA and RNA targets, fluorescence genotyping, CGH microarray, spectral karyotyping/multicolor FISH, and primed in situ labeling. There are also techniques for multicolor fiber FISH, multi-telomere FISH, prenatal diagnosis using maternal blood, and preimplantation diagnosis. Oncological methods include simultaneous fluorescence immunophenotyping and FISH for leukemia and lymphoma, HER2 amplification in breast cancer, and CAC/PAC for cancer cytogenetics.

Table of Contents
Part I. Basic Concepts and Techniques

Molecular Cytogenetics in Medicine: An Overview
Yao-Shan Fan

Labeling Fluorescence In Situ Hybridization Probes for Genomic Targets
Larry E. Morrison, Ramesh Ramakrishnan, Teresa M. Ruffalo, and Kim A. Wilber

Labeling Fluorescence In Situ Hybridization Probes for RNA Targets
Ramesh Ramakrishnan and Larry E. Morrison

Basic FISH Techniques and Troubleshooting
Sue Van Stedum and Walter King

Part II. Evolving Techniques and Applications

Chromosome Microdissection
Xin-Yuan Guan

Primed In Situ Labeling
J酶rn Koch

Spectral Karyotyping
Jane Bayani and Jeremy A. Squire

Multicolor FISH
Syed M. Jalal and Mark E. Law

Characterization of Constitutional Chromosome Abnormalities by Comparative Genomic Hybridization
Brynn Levy and Kurt Hirschhorn

Color Banding
Vicki L. Hopwood, Christi J. Hada, and Armand B. Glassman

Multicolor Fiber FISH
Jaennelle Kraan, Anne R. M. von Bergh, Karin Kleiverda, Jan-Willem Vaandrager, Ekaterina S. Jordanova, Anton K. Raap, Philip M. Kluin, and Ed Schuuring

Multi-Telomere FISH
Samantha J. L. Knight and Jonathan Flint

Fluorescence Genotyping for Screening Cryptic Telomeric Rearrangements
Laurence Colleaux, Solange Heuertz, Florence Molinari, and Marlene Rio

Microarray CGH, Ben Beheshti, Paul C. Park, Ilan Braude, and Jeremy A. Squire

Part III. Special Applications in Chromosomal Disorders

Molecular Approaches for Delineating Marker Chromosomes
Michael D. Graf and Stuart Schwartz

Interphase FISH for Prenatal Diagnosis of Common Aneuploidies
Baruch Feldman, Ayala Aviram-Goldring, and Mark I. Evans

Prenatal Diagnosis of Chromosomal Abnormalities Using Maternal Blood, Farideh Z. Bischoff, Dianne X. Dang, Dorothy E. Lewis, and Joe Leigh Simpson

Preimplantation FISH Diagnosis of Aneuploidies
Yury Verlinsky, Jeanine Cieslak, and Anver Kuliev

Microdeletion Syndromes: Characteristics and Diagnosis
Stuart Schwartz and Michael D. Graf

Molecular Detection of Uniparental Disomy
Wendy P. Robinson

Molecular Cytogenetics in Reproductive Pathology
H茅l谩?e Bruy谩?e, Evica Rajcan-Separovic, and Dagmar K. Kalousek

Part IV. Special Applications in Oncology

Interphase FISH Studies of Chronic Myeloid Leukemia, Gordon W. Dewald

Chromogenic In Situ Hybridization and FISH in Pathology
Bae-Li Hsi, Sheng Xiao, and Jonathan A. Fletcher

FISH Detection of HER2 Amplification in Breast Cancer
Rosemary E. Mueller and Frances P. O'Malley

Comparative Genomic Hybridization in Cancer Investigations
Marjan M. Weiss, Mario A. J. A. Hermsen, Gerrit A. Meijer, and Paul J. van Diest

Simultaneous Fluorescence Immunophenotyping and FISH on Tumor Cells
Yanming Zhang and Brigitte Schlegelberger

BAC Resource for Molecular Cytogenetics
Xiao-Ning Chen and Julie R. Korenberg

Index

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