Flow Cytometry Protocols 第2版

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Flow Cytometry Protocols: Second Edition

Humana Press

Author(s): Hawley, Teresa S. and Hawley, Robert G.

eBook $99.50

Series: Methods in Molecular Biology  
Pub Date:  March 2004  
Pages: 448
ISBN: 1-58829-234-7  







Table of Contents  
1 Flow Cytometry: An Introduction
Alice L. Givan

2 Multiparameter Flow Cytometry of Bacteria
Howard M. Shapiro and Gerhard Nebe-von-Caron

3 Multiparameter Data Acquisition and Analysis of Leukocytes
Carleton C. Stewart and Sigrid J. Stewart

4 Flow Cytometric Analysis of Kinase Signaling Cascades
Omar D. Perez, Peter O. Krutzik, and Garry P. Nolan

5 Cytokine Flow Cytometry
Holden T. Maecker

6 Use of Cell-Tracking Dyes to Determine the Proliferation Precursor Frequencies of Antigen-Specific T Cells
Alice L. Givan, Jan L. Fisher, Mary G. Waugh, Nadège Bercovici, and Paul K. Wallace

7 Assessment of Lymphocyte-mediated Cytotoxicity Using Flow Cytometry
Luzheng Liu, Beverly Z. Packard, Martin J. Brown, Akira Komoriya, and Mark B. Feinberg

8 Multiparametric Analysis of Apoptosis by Flow and Image Cytometry
William G. Telford, Beverly Z. Packard, and Akira Komoriya

9 Detection and Enrichment of Hematopoietic Stem Cells by Side Population Phenotype
Shannon S. Eaker, Teresa S. Hawley, Ali Ramezani, and Robert G. Hawley

10 Hematopoietic Stem Cell Characterization by Hoechst 33342 and Rhodamine 123 Staining
Ivan Bertoncello and Brenda Williams

11 Phenotypic and Functional Analyses of Human CD34NEG Hematopoietic Precursors from Mobilized Peripheral Blood
Douglas C. Dooley and Barbara K. Oppenlander

12 Multiparameter Flow Cytometry of Fluorescent Protein Reporters
Teresa S. Hawley, Donald J. Herbert, Shannon S. Eaker, and Robert G. Hawley


13 The Analysis of Fluorescent Protein Expressing Cells by Flow Cytometry
Steven C. Pruitt, Lawrence M. Mielnicki, and Carleton C. Stewart

14 Integrative Flow Cytometric and Microarray Approaches for Use in Transcriptional Profiling
David W. Galbraith, Rangasamy Elumalai, and Fang Cheng Gong

15 Flow Cytometric Analysis of Fluorescence Resonance Energy Transfer: A Tool for High-throughput Screening of Molecular Interactions in Living Cells
Francis Ka-Ming Chan and Kevin L. Holmes

16 Design of a FACS-based Mammalian Protein-Protein Interaction Trap (MAPPIT)
Sam Lievens, José Van der Heyden, Els Vertenten, Jean Plum, Jo雔 Vandekerckhove, and Jan Tavernier

17 Flow Cytometric Screening of Yeast Surface Display Libraries
Michael Feldhaus and Robert Siegel

18 FRET-based HIV-1 Virion Fusion Assay
Marielle Cavrois, Jason Neidleman, Martin Bigos, and Warner C. Greene

19 Cell Cycle Analysis of Asynchronous Populations
Michael G. Ormerod

20 Solid Tumor DNA Content Analysis
Adel K. El-Naggar and Philippe Vielh

21 Concurrent Flow Cytometric DNA and RNA Analysis
Adel K. El-Naggar

22 Telomere Length Measurement by FISH and Flow Cytometry
Veena Kapoor and William G. Telford

23 Small Lasers in Flow Cytometry
William G. Telford

24 Viable Infectious Cell Sorting in a BSL-3 Facility
Stephen P. Perfetto, David R. Ambrozak, Mario Roederer, and Richard A. Koup