Lentivirus Gene Engineering Protocols (Methods in Molecular Biology (Hardcove

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ISBN: 1588290913
Title: Lentivirus Gene Engineering Protocols (Methods in Molecular Biology (Hardcover))
Author:
Publisher: Humana Press
Publication Date: 2003-05
Number Of Pages: 314
Average Amazon Rating:
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Methods in Molecular Biology, vol.229

Maurizio Federico has assembled a panel of outstanding experimenters to detail all the theoretical and practical aspects of lentivirus vector-based gene transfer. The authors demonstrate lentivirus vector production with methods for recovering appropriate producer cells, for producing and titrating lentivirus-containing supernatants, and for detecting transduced cells. The applications of lentivirus vector engineering to different cell types include coverage of lymphocytes, dendritic cells, hematopoietic stem and progenitor cells, mesenchymal stem cells, hippocampal neurons, cardiomyocytes, as well as airway epithelia, corneal cells, and retinal pigment. Also advanced are applications in which genetic material is directly inoculated with lentivirus vectors in such experimental animal models as mouse, rat, and rabbit.




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