Therapeutic Proteins: Methods and Protocols

yujian623

Publisher: Humana Press
Number Of Pages: 496
Publication Date: 2005-08-15
Sales Rank: 526563
ISBN / ASIN: 1588293904
EAN: 9781588293909
Binding: Hardcover
Manufacturer: Humana Press
Studio: Humana Press

Book Description:
World-class researchers share their innovative and proven techniques for the production of therapeutic proteins downstream of the discovery stage. The methods use a variety of sources for producing therapeutic proteins, including bacterial and yeast expression systems, and insect and mammalian cells, and then cover the purification of the resulting protein using both state-of-the-art and traditional methods, such as those sourced from plasma. Additional chapters offer methods for the characterization of therapeutic proteins throughout the production process, along with methods and strategies for viral inactivation and protein formulation. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

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