Principles and Protocols (Methods in Molecular Biology, Volume 148)

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Book Properties
ISBN: 0896036715
Title: Dna-Protein Interactions: Principles and Protocols (Methods in Molecular Biology, Volume 148)
Author: Tom Moss
Publisher: Humana Press
Publication Date: 2001-04-15
Number Of Pages: 638

General description
Revised second edition provides the only book exclusively concentrating on up-to-date techniques for protein-DNA interactions. Easy-to-follow protocols illuminate the molecular basis of protein-nucleic acid interactions. Use them successfully to reveal location of DNA binding site, strength and specificity of a binding, identities of individual groups on actual bases involved in binding, and specific amino acid residues of the protein that interact with DNA. Some techniques can be used to identify previously unknown DNA binding proteins from crude cell extracts.

Dr. Tom Moss assembles the new standard collection of cutting-edge techniques to identify key protein