发一个有用的蛋白质提取等相关资源

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EXPRESSION SYSTEMS
EXTRACTION AND CLARIFICATION
PURIFICATION CHARACTERIZATION

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EXPRESSION SYSTEMS

Bacterial Expression Systems

        GST-Fusion Proteins
        PHARMACIA-GST fusion protein Handbook (pdf)
        poly His-Tagged Fusion Proteins
        CLONTECH:  BD HAT Protein Expression and Purification System User Manual
        NOVAGEN:  pET purification manual  (pdf)
        QIAGEN: Ni-NTA purification manual (pdf)
        Maltose Binding Protein
        NEW ENGLAND BIOLAB: pMAL protein Fusion and Purification System (pdf)
        Calmodulin Binding Peptide
        STRATAGENE: CBP Calmodulin-Binding Peptide Affinity Tag System
        Intein Mediated Purification
        NEW ENGLAND BIOLAB: INTEIN-TM System (pdf)
        NEW ENGLAND BIOLAB: INTEIN-TWIN System (pdf)

Biotinylated Fusion Protein

           PROMEGA   PinPointTM Xa Protein Purification System  (pdf)   

      Two Step: poly His and Strep-Tag
           QIAGEN:  Two-Step Affinity Purification System Handbook
                         For expressing, purifying, and detecting proteins carrying a 6xHis and Strep-tag II  (pdf)

        SUMO


          LIFESENSORS  SUMOpro™ Expression System T7, Amp, E.coli  (pdf)   
          LIFESENSORS  SUMOpro™ Expression System  T7, Kan, E.coli  (pdf)
          LIFESENSORS  SUMO Gene Fusion Technology - New Methods for Enhancing Protein Expression and Purification in Eukaryotes  (pdf)
          LIFESENSORS  SUMO Gene Fusion Technology - New Methods for Enhancing Protein Expression and Purification in Prokaryotes  (pdf)
          LIFESENSORS  SUMO Gene Fusion Technology - New Methods for Rapid Recombinant Peptide Expression and Purification  (pdf)






Drosophila Expression System


         INVITROGEN :  Drosophila Expression System  (pdf)


Eukaryote Expression System


           LIFESENSORS  Split SUMOpro™ Expression System CUP1, Amp, Yeast  (pdf)   
           LIFESENSORS  Split SUMOpro™ Expression System pPolh, Amp, Insect  (pdf)


In-Vitro Translation System

           ROCHE Rapid Translation System Products
           ROCHE Rapid Translation System RTS100  (pdf)
           ROCHE_Rapid Translation System RTS500  (pdf)
           ROCHE_Rapid Translation System RTS500 Circular  (pdf)
           ROCHE_Rapid Translation System RTS9000  (pdf)
           ROCHE_RTS_Amino Acid Sampler  (pdf)
           ROCHE_Rapid Translation System GroE Supplement  (pdf)


Insect Expression System


           NOVAGEN  InsectDirect™ Protein Expression & Purification System  (pdf)


Leishmania  

            JENA  BIOSCIENCE  LEXSY  Leishmania tarentolae Expression System  (pdf)  (pdf-article)

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Preparation of Cell Lysates / Different Disruption Methods

     NOVAGENBugBaster Bacterial Protein Extraction (pdf)
     NOVAGEN :  Lysonase Bioprocessing Reagent  (pdf)
     NOVAGEN  POP culture Reagent  (pdf)
     PIERCE  B-Per Bacterial Protein Extraction Reagent  (pdf)
     Bacterial Protein Extraction (mini-scale) Sonication
     Bacterial Protein Extraction (mini-scale) Using B-Per
     PROTEIN EXPRESSION FACILITY of THE HEBREW UNIVERSITY Sonication of bacterial samples
  

Choice of Buffers
  AMERSHAM BIOSCIENCES  Recommended buffers for Anion Exchange Chromatography
    AMERSHAM BIOSCIENCES  Recommended buffers for Cation Exchange Chromatography
    AMERSHAM BIOSCIENCES  Recommended Volatile buffers systems for Ion Exchange Chromatography
    AMRESCO Buffers Technical Bulletin  (pdf)
    CALBIOCHEM Buffers - A guide for the preparation and use of buffers in biological systems  (pdf)
    CALBIOCHEM Table of detergents and buffers  (pdf)
    PROTEIN EXPRESSION AND PURIFICATION FACILITY OF THE EUROPEAN MOLECULAR BIOLOGY LABORATORY  Choice of lysis buffer and additives
    PROTEIN EXPRESSION AND PURIFICATION FACILITY OF THE EUROPEAN MOLECULAR BIOLOGY LABORATORY  Solubility Studies
Preparation of soluble/insoluble protein from cells
    SIGMAUseful pH Ranges of Selected Biological Buffers (pdf)
  

Inhibitors

Apoptosis Inhibitors


    CALBIOCHEM  Calbiochem Inhibitor SourceBook  (pdf)


Cell Division/Cell Cycle/Cell Adhesion Inhibitors


    CALBIOCHEM  Calbiochem Inhibitor SourceBook  (pdf)


Lipid Signal Inhibitors


    CALBIOCHEM  Calbiochem Inhibitor SourceBook  (pdf)


Neurobiology/Neurodegeneration Inhibitors


    CALBIOCHEM  Calbiochem Inhibitor SourceBook  (pdf)


Nitric Oxid/Oxidative Stress Inhibitors


    CALBIOCHEM  Calbiochem Inhibitor SourceBook  (pdf)


Phosphorilation/Dephosphorilation


    CALBIOCHEM  Calbiochem Inhibitor SourceBook  (pdf)


Protease Inhibitors

    CALBIOCHEM  Calbiochem Inhibitor SourceBook  (pdf)
    CALBIOCHEM Protease Inhibitor (pdf)
    CALBIOCHEM Protease Inhibitor (pdf)   (pdf-II)
    ROCHE  Protease Inhibitor (pdf)
    ROCHE  Universal Protease Substrate  (pdf)
    SERVA  Protease Inhibitors  (pdf)

Others


    CALBIOCHEM  Calbiochem Inhibitor SourceBook  (pdf)


DNA removal
   NOVAGEN Benzonase Nuclease - Removal of Nucleic Acids (pdf)
      NOVAGEN :  Lysonase Bioprocessing Reagent  (pdf)
      Removal of Nucleic Acids - Different Protocols
      VIVASCIENCE  Removal of DNA from lysates of E. coli cells and French bean tissue prior to electrophoresis.  (pdf)


Endotoxin removal
      BIORAD   Affi-Prep

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Purification Strategy - Others

    AMERSHAM-BIOSCIENCES  Handbook Protein Purification  (pdf)   
     AMERSHAM-BIOSCIENCES  Protein purification Applications  (pdf)
      BIO RAD Sample Preparation Handbook  (pdf)
      CLONTECH:  Troubleshooting Guide  (pdf)
      Test Tube      
      MERCK   Cleaning and Regeneraton of  Fractogel EMD sorbents  (pdf)
      NOVAGEN  Benzonase Nuclease - Removal of Nucleic Acids (pdf)
      NOVAGENBuffer Compositin for Fusion Tag Affinity Purification (pdf)
     Removal of Nucleic Acids - Various Protocols   
      





Purification of Recombinant Proteins


    AMERSHAM-BIOSCIENCES   The Recombinant Protein Handbook  (pdf)  
    PIERCE:  Fusion Protein Fusion Protein Products  (pdf)
    SIGMA:  DETECTION AND PURIFICATION  Detection and Purification Selection Guide  (pdf-I)   (pdf-II)


GST-Fusion Proteins
    CLONTECH:  Glutathione Resins  (pdf)
      CLONTECH:  GST products Handbook (pdf)

      CLONTECH:  Protein Purification Products  (pdf)
      AMERSHAM-BIOSCIENCES  GST fusion protein Handbook  (pdf)
      NOVAGENGST.Bind Kits(pdf)
      NOVAGEN: His-Tag GST-Tag purification and Detection tools  (pdf)
      Small scale GST-fusion protein purification under nature conditions


poly His-Tagged Fusion Proteins

     NOVAGEN:  pET purification manual  (pdf)
      NOVAGEN: Ni-NTA Hi-Bind Resins Protocols  (pdf)
      NOVAGEN: His-Tag GST-Tag purification and Detection tools  (pdf)
      QIAGEN: Ni-NTA purification manual (pdf)
      QIAGEN: NiNTA cell lysis under nature conditions (pdf)
      MERCK:  Metal Chelate Affinity Chromatography  (pdf)  (pdf-II)
      CLONTECH: Talon Resin Protocol (pdf)
      CLONTECH: Talon Products (pdf)
      CLONTECH:  BD TalonTM Metal Affinity Resins User Manual  (pdf)
      CLONTECH:  Purification in the presence of Beta Mercaptoethanol  (pdf)
      CLONTECH:  Protein Purification Products  (pdf)
      CLONTECH:  BD HAT Protein Expression and Purification System User Manual  (pdf)
      AMERSHAM-BIOSCIENCES Hi Trap Chelating (pdf)
      AMERSHAM-BIOSCIENCES Hi Trap Chelating Application Note  (pdf)
      AMERSHAM-BIOSCIENCES  Chelating Sepharose Fast Flow Instructions  (pdf)
      AMERSHAM-BIOSCIENCES    Ni Sepharose High Performance  (pdf)  (pdf-II)
      ACTIVE MOTIVE: Ni-Ted Manual  (pdf)
      AFFILAND: Metal Purification
      VIVASCIENCE:  Vivapure Metal Chelate Mini Spin Columns  (pdf-I)  (pdf-II)  (pdf-III)  (pdf-IV)  (pdf-V)  (pdf-VI)
      Small scale His-Tag fusion protein purification under nature conditions
      Small scale His-Tag fusion protein purification under denaturative conditions
      DYNAL  Dynabeads TalonTM  (pdf)
      Ni-NTA: Handling and Reuse
      SIGMA:  His Selected Nickel Affinity Gel  (pdf)
      PROMEGA:  MagneHisTM Protein Purification System  (pdf)      
      HISPANAGAR  Chelate Agarose Beads  (pdf-I)  (pdf-II)  (pdf-III)   
      SARTORIUS  Sartobind

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Protein Quantitation    Comparision of Different Protein Determination Methods
    Absorbance 280nm According toProtein Protocols in CD Rom
    Bradford   
    Lowry  According toProtein Protocols in CD Rom
    Lowry-Peterson  For membrane proteins, diluted solutions or solutions with interferants   
    Protein Precipitation Methods to concentrate or eliminate interferences before electrophoresis or protein determination

    BIO-RAD   Bradford Modified BIO RAD protein Assay  (pdf)
    BIO-RAD   Lowry Modified DC BIO-RAD (pdf)   (pdf-2)
    BIO-RAD   Lowry Modified RC DC BIO-RAD (pdf)
    GENO  Non-Interfering Protein Assay GENO Technology


    MOLECULAR PROBES   CBQCA Protein Quantitation Kit    (pdf)
    MOLECULAR PROBES   Nano Orange  (pdf)
    MOLECULAR PROBES   Quant-iTTM Protein Assay Kit  (pdf)
    MOLECULAR PROBES   FluoReporter

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